The antiserum was produced against synthesized peptide derived from human CAD around the phosphorylation site of Thr456.
Immunogen Range
422-471
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:5000
Specificity
CAD (Phospho-Thr456) Antibody detects endogenous levels of CAD only when phosphorylated at Thr456.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CAD
Database Links
Gene ID: 790 Swiss-Prot #: P27708
OMIM #: 114010 Unigene #: Hs.377010
Target Modification
Phospho
Modification Sites
H:T456 M:T456
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using CAD (Phospho-Thr456) Antibody. The picture on the right is blocked with the phospho peptide.