Immunogen | KLH-conjugatedlinearpeptidecorrespondingtomouseTbr2. |
Epitope | TranscriptionActivationDomain |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Chicken |
Specificity | ExpectedtoreactwithbothsplicedisoformsofmurineTbr2. |
Isotype | IgY |
SpeciesReactivity | |
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
GeneSymbol | |
PurificationMethod | AffinityPurfied |
UniProtNumber | |
UniProtSummary | FUNCTION:Functionsasatranscriptionalactivatorplayingacrucialroleduringdevelopment.Functionsintrophoblastdifferentiationandlateringastrulation,regulatingbothmesodermdelaminationandendodermspecification.PlaysaroleinbraindevelopmentbeingrequiredforthespecificationandtheproliferationoftheintermediateProgenitorcellsandtheirprogenyinthecerebralcortex.AlsoinvolvedinthedifferentiationofCD8+T-cellsduringimmuneresponseregulatingtheexpressionoflyticeffectorgenes.
SUBCELLULARLOCATION:NucleusProbable.
TISSUESPECIFICTY:ExpressedinCD8+T-cells.
DEVELOPMENTALSTAGE:Originallyexpressedinthetrophoectodermoftheblastocystandlaterintheextra-embryonicectodermoftheearlypost-implantationembryo.Intheembryoproper,expressedintheposteriorpartoftheepIBLast.Duringgastrulation,extendsdistallyintotheprimitivestreakandnascentmesoderm.Alsoexpressedinthedevelopingforebrainandtheolfactorylobes.ExpressedatE12.5andE14.5intheforebrain.
INDUCTION:Up-regulatedinCD8+T-cellsuponactivation.
DISRUPTIONPHENOTYPE:Embryoniclethalduetoperi-implantationdefects.Mutantembryosarrestsoonafterimplantationandfailtoformorganizedembryonicorextraembryonicstructures.Conditionalmutants,withexpressionabrogatedintheinnercellmassofembryosfromearlyimplantationstagesonward,displaygastrulationdefects.
SEQUENCESIMLARITIES:Contains1T-boxDNA-bindingdomain. |
MolecularWeight | ~85/95kDaobserved.Targetbandsappearlargerthanthecalculatedmolecularsizeof74.8kDa(isoform1)and72.7kDa(isoform2). |