Immunogen | Syntheticpeptidecorrespondingtoaminoacids41-56ofhumancaspase-9(Duanetal.1996;Srinivasulaetal.1996;Nijhawanetal.1997).Recognizesproform(46kDa);sequenceanalysispredictscross-reactivitywithmousecaspase-9(>90%). |
Epitope | proform&lg.subunit |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Rabbit |
Specificity | Apoptosisisrelatedtomanydiseasesandinducedbyafamilyofcelldeathreceptorsandtheirligands.Celldeathsignalsaretransducedbydeathdomaincontainingadaptermoleculesandmembersofthecaspasefamilyofproteases.AnovelmemberinthecaspasefamilywasrecentlyidentifiedanddesignatedICE-LAP6,Mch6,andApaf-3(Duanetal.1996;Srinivasulaetal.1996;Nijhawanetal.1997).Caspase-9andApaf-1bindtoeachother,whichleadstocaspase-9activation(Nijhawanetal.1997).Caspase-9isalsoactivatedbygranzymeBandCPP32(Duanetal.1996;Srinivasulaetal.1996).Activatedcaspase-9cleavesandactivatescaspase-3thatisoneofthekeyproteases,beingresponsIBLefortheproteolyticcleavageofmanykeyproteinsinapoptosis(Nijhawanetal.1997).Caspase-9playsacentralroleincelldeathinducedbyawidevarietyofapoptosisinducersincludingTNF-alpha,TRAIL,anti-CD-95,FADD,andTRADD(Panetal.1998).Caspase-9isexpressedinavarietyofhumantissues(Duanetal.1996;Srinivasulaetal.1996). |
SpeciesReactivity | |
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Thisgeneencodesamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.ThisproteinisprocessedbycaspaseAPAF1;thisstepisthoughttobeoneoftheearliestinthecaspaseactivationcascade.Alternativesplicingresultsintwotranscriptvariantswhichencodedifferentisoforms. |
GeneSymbol | - CASP9
- CASP-9
- ICE-LAP6
- APAF3
- MCH6
- CASPASE-9c
- OTTHUMP00000044594
- APAF-3
- EC3.4.22.62[Contains:Caspase-9subunitp35
- Caspase-9subunitp10].
|
Non-ReactiveSpecies | |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P55211#Isoform2lacksactivityisandominant-negativeinhibitorofcaspase-9. SIZE:416aminoacids;46281Da SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya35kDa(p35)anda10kDa(p10)subunit.Caspase-9andAPAF1bindtoeachotherviatheirrespectiveNH2-terminalCED-3homologousdomainsinthepresenceofcytochromeCandATP.InteractswiththeinhibitorsBIRC2,BIRC4,BIRC5andBIRC7. TISSUESPECIFICITY:Ubiquitous,withhighestexpressionintheheart,moderateexpressioninliver,skeletalmuscle,andpancreas.Lowlevelsinallothertissues. PTM:CleavagesatAsp-315bygranzymeBandatAsp-330bycaspase-3generatethetwoactivesubunits.Caspase-8and-10canalsobeinvolvedintheseprocessingevents. SIMILARITY:SwissProt:P55211##BelongstothepeptidaseC14family.&Contains1CARDdomain. |