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Millipore/AB5898 | Anti-Protein Gene Product 9.5 Antibody/AB5898/50 µL
  • Millipore/AB5898 | Anti-Protein Gene Product 9.5 Antibody/AB5898/50 µL

Millipore/AB5898 | Anti-Protein Gene Product 9.5 Antibody/AB5898/50 µL

價(jià)格: ¥4308.00 市場(chǎng)價(jià): 7180.00

貨號(hào): AB5898
品牌: Millipore
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    • Description
      CatalogueNumberAB5898
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-ProteinGeneProduct9.5Antibody
      AlternateNames
      • PGP9.5
      • UCH-L1
      ProductInformation
      FormatSerum
      Control
      • Braintissue
      PresentationLiquid.Contains0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationAnti-ProteinGeneProduct9.5AntibodydetectslevelofProteinGeneProduct9.5&hasbeenpublished&validatedforuseinIH.
      KeyApplications
      • Immunohistochemistry
      ApplicationNotesImmunohistochemistry:1:500.

      Optimalworkingdilutionsmustbedeterminedbyenduser.

      blockingbufferfor1houratroomtemperature.Primaryantibodywasdilutedinblockingbuffertotheappropriateworkingdilution.Blockingbufferwasremovedandtheslideswerethenincubatedat4°Cfor18-24hourswithAB5898(1:500).AfterrinsinginPBS3timessectionswereincubatedfor60minutesatroomtemperaturewithCy3-conjugatedsecondaryantibodies.AftermountinginamixtureofPBSandglycerol(1:3)containing0.1%p-phenylenediamine,sectionswereexaminedwithaNikonMicrophot-SAepifluorescencemicroscope.
      BIOLOGicalInformation
      ImmunogenA17aminoacidpeptidecorrespondingtoaminoacids175-191fromsolublecytoplasmichumanPGP9.5.
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostGuineaPig
      SpecificityRecognizesProteinGeneProduct9.5(PGP9.5).
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryUCHL1isamemberofagenefamilywhoseproductshydrolyzesmallC-terminaladductsofubiquitintogeneratetheubiquitinmonomer.ExpressionofUCHL1ishighlyspecifictoneuronsandtocellsofthediffuseneuroendocrinesystemandtheirtumors.Itispresentinallneurons(Doranetal.,1983[PubMed6343558]).[suppliedbyOMIM]
      GeneSymbol
      • UCHL1
      • PARK5
      • UCH-L1
      • Uch-L1
      • PGP9.5
      • EC3.4.19.12
      • EC6.-.-.-
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P09936#Ubiquitin-proteinhydrolaseinvolvedbothintheprocessingofubiquitinprecursorsandofubiquitinatedproteins.ThisenzymeisathiolproteasethatrecognizesandhydrolyzesapeptidebondattheC-terminalglycineofubiquitin.Alsobindstofreemonoubiquitinandmaypreventitsdegradationinlysosomes.ThehomodimermayhaveATP-independentubiquitinligaseactivity.
      SIZE:223aminoacids;24824Da
      SUBUNIT:Homodimer.InteractswithSNCA(Bysimilarity).InteractswithCOPS5.
      SUBCELLULARLOCATION:Cytoplasm.
      TISSUESPECIFICITY:Foundinneuronalcellbodiesandprocessesthroughouttheneocortex(atproteinlevel).Expressedinneuronsandcellsofthediffuseneuroendocrinesystemandtheirtumors.Weaklyexpressedinovary.
      PTM:O-glycosylated(Bysimilarity).
      DISEASE:SwissProt:P09936#OxidationofMet-1,Met-6,Met-12,Met-124andMet-179tomethioninesulfoxide,andoxidationofCys-220tocysteinesulfonicacidhavebeenobservedinbrainsfromAlzheimerdisease(AD)andParkinsondisease(PD)patients.InAD,UCHL1wasfoundtobeassociatedwithneurofibrillarytangles.
      SIMILARITY:SwissProt:P09936##BelongstothepeptidaseC12family.
      MISCELLANEOUS:IncontrasttoUCHL3,doesnothydrolyzeapeptidebondattheC-terminalglycineofNEDD8.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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