The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Smad3 Antibody
Synonyms
JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3; Mothers against decapentaplegic homolog 3; SMAD 3; Smad 3
Immunogen
The antiserum was produced against synthesized peptide derived from human Smad3.
Immunogen Range
170-219
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
Smad3 Antibody detects endogenous levels of total Smad3 protein.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
SMAD3
Database Links
Gene ID: 4088 Swiss-Prot #: P84022
OMIM #: 114500/603109 Unigene #: HS.36915
Target Modification
Unmodified/Total
Modification Sites
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Smad3 Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Western blot analysis of lysates from HUVEC cells, using Smad3 Antibody. The lane on the right is blocked with the synthesized peptide.