The antiserum was produced against synthesized peptide derived from human eNOS.
Immunogen Range
1145-1194
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000
Specificity
eNOS Antibody detects endogenous levels of total eNOS protein.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NOS3
Database Links
Gene ID: 4846 Swiss-Prot #: P29474
OMIM #: 163729 Unigene #: Hs.647092
Target Modification
Unmodified/Total
Modification Sites
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using eNOS Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Western blot analysis of lysates from A549, K562, and COLO205 cells, using eNOS Antibody. The lane on the right is blocked with the synthesized peptide.