Phenylethynylpyrene(PEP)fluorophoreisapolyaromatichydrocarbonlabelwithhighsensitivitytomicroenvironment.Similarlytopyrene,PEPdyereADIlyformsexcimers.However,PEPfluorescenceismorered-shifted
PEPcanbeusedasmicroenvironmentprobe,andasalabelforassaysbasedonexcimerformation.
Thisreagentcontainstriethyleneglycollinkertofacilitatedissolutionofnon-polarPEPdyeinorgano-aqueouslabelingreactionmixtures.Withthisazide,andClickchemistry,itiseasytoturnanymoleculebearingalkyneintoPEP-labeledprobe.
Generalproperties
Appearance: | offwhite/yellowishsolid |
Molecularweight: | 544.64 |
Molecularformula: | C34H32N4O3 |
Solubility: | solubleindichloromethane,chloroform,moderatelysolubleinDMSO,DMF,acetonitrile |
Qualitycontrol: | NMR1H(95%) |
Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight. |
Spectralproperties
Excitationmaximum,nm: | 293;362;384 |
Emissionmaximum,nm: | 389 |