The antiserum was produced against synthesized peptide derived from human eIF4E.
Immunogen Range
168-217
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
eIF4E Antibody detects endogenous levels of total eIF4E protein.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
EIF4E
Database Links
Gene ID: 1977 Swiss-Prot #: P06730
OMIM #: 133440 Unigene #: Hs.249718
Target Modification
Unmodified/Total
Modification Sites
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using eIF4E Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Western blot analysis of lysates from NIH/3T3 cells, treated with FBS, using eIF4E Antibody. The lane on the right is blocked with the synthesized peptide.