CollagenHybridizingPeptide(CHP)islabeledwitheithercarboxyfluorescein(Cat#:C-660F)forfluoroscencedetectionorbiotin(Cat#:C-660B)foravidin/streptavidin-mediateddetection.Whenusedonminimallyprocessedtissuesamples(e.g.,cryosections),itcanvisualizethepresenceofdenaturedcollageninpathologicaltissues[2].Itcanalsobeusedinplaceoffailedcollagenantibodiestodetectcollagenonhighlyprocessedtissuesamples[3].Inaddition,CHPallowsdirectandspecificdetectionofcollagenbandsinSDS-PAGEgelswithouttheneedofwesternblot[3]. Storage-20°CReferences1)Li,YandYuSM“Targetingandmimickingcollagensviatriplehelicalpeptideassemblies”CurrOpinChemBiol,2013,17,968-975. 2)LiY,FossCA,SummerfieldDD,DoyleJJ,TorokCM,DietzHC,PomperMG,andYuSM“Targetingcollagenstrandbyphoto-triggeredtriple-helixhybridization”P(pán)roceedingsoftheNationalAcademyofSciencesoftheUnitedStatesofAmerica,2012,109,14767-14772.DOI:10.1073/pnas.1209721109. 3)LiY,HoD,HuanM,ChanTR,AnB,YuH,BrodskyB,JunAS,andYuSM“Directdetectionofcollagenousproteinsbyfluorescentlylabeledcollagenmimeticpeptides”BioconjugateChemistry,2013,24,9-16.DOI:10.1021/bc3005842.RelatedProductsCollagenIVELISAKitII(K-1700s) |