Description:
NEB10-betaelectrocompetentE.colicellsareoptimizedforhighefficiencytransformationbyelectroporation.ThesecellsareidealforDNAlibraryconstructionsandallcloningpurposes.
Highlights
- DH10B?derivative
- Transformationefficiency:2x1010cfu/μgpUC19
- AccomodationoflargeplasmidsincludingBACandcosmidconstructs
- EfficienttransformationofmethylatedDNAderivedfromeukaryoticsourcesandunmethylatedDNAderivedfromPCR,CDNAandothersources
- ActivityofnonspecificendonucleaseIeliminatedforhighestqualityplasmidpreparations(endA1)
- Suitableforblue/whitescreeningbyα-complementationoftheβ-galactosidasegene(Φ80ΔlacZM15)
- ReducedrecombinationofclonedDNA(recA)
- ResistancetophageT1lytic(fhuA2)
- K-12Strain
- Freeofanimalproducts
Genotype
Δ(ara-leu)7697araD139?fhuAΔlacX74galK16galE15e14-?Φ80dlacZΔM15?recA1relA1endA1nupG?rpsL(StrR)rphspoT1Δ(mrr-hsdRMS-mcrBC)?
ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
pUC19Vector | -20 | 0.05ng/μl |
NEB®10-beta/StableOutgrowthMedium | 4 | |
Notes:
STORAGEANDHANDLING:Competentcellsshouldbestoredat-80°C.Storageat-20°Cwillresultinasignificantdecreaseintransformationefficiency.Cellsloseefficiencywhenevertheyarewarmedabove-80°C,eveniftheydonotthaw.