EML4 Split CISH Probe is designed for the qualitative detection of translocations involving the human EML4 gene at 2p21 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Recommend Usage:
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
Supplied Product:
Reagent Provided:This Probe is composed of:1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 2p21* (chr2:42,342,038-42,464,761) distal to the EML4 breakpoint region.2. Dinitrophenyl-labeled polynucleotides, which target sequences mapping in 2p21* (chr2:42,576,262-43,163,545) proximal to the EML4 breakpoint region. 3. Formamide based hybridization buffer.*according to Human Genome Assembly GRCh37/hg19
Storage Instruction:
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note:
The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.Interpretation of results:Using the CISH Implementation Kit 2 (Cat # KA5366), hybridization signals of Digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (distal to the EML4 breakpoint region), and Dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (proximal to the EML4 breakpoint region).Normal situation: In interphases of normal cells or cells without a translocation involving the EML4 gene region, two red/green fusion signals appear.Aberrant situation: One EML4 gene region affected by a translocation is indicated by one separate green signal and one separate red signal.Overlapping signals may appear as brown signals. Genomic aberrations due to small deletions, duplications or inversions might result in inconspicuous signal patterns. Other signal patterns than those described above may be observed in some abnormal samples. These unexpected signal patterns should be further investigated.
Probe Position:
Regulatory Status:
For research use only (RUO)
Interpretation of Result:
Datasheet:
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Applications
Chromogenic In Situ Hybridization (Cells)
EML4 Split CISH Probe hybridized to normal interphase cells as indicated by two red/green fusion signals pert nucleus.
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