RET Split CISH Probe is designed for the qualitative detection of human RET gene at 10q11.21 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Recommend Usage:
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
Supplied Product:
Reagent Provided:1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 10q11.21* (chr10:43,687,278-43,856,587) distal to the RET breakpoint region2. Dinitrophenyl-labeled polynucleotides targeting sequences mapping in 10q11.21* (chr10:43,340,888-43,510,171) proximal to the RET breakpoint region 3. Formamide based hybridization buffer*according to Human Genome Assembly GRCh37/hg19
Storage Instruction:
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note:
The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.Hybridization signals of digoxigenin-labeled polynucleotides appear dark green colored distinct dotshaped (distal to the RET breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (proximal to the RET breakpoint region)).Normal situation: In interphases of normal cells or cells without a translocation involving the RET gene region, two red/green fusion signals appear.Aberrant situation: One RET gene region affected by a translocation is indicated by one separate green signal and one separate red signal. Isolated green signals are the result of deletions proximal to the RET breakpoint region. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements. Unexpected signal patterns should be further investigated.
Probe Position:
Regulatory Status:
For research use only (RUO)
Interpretation of Result:
Datasheet:
Download
Applications
Chromogenic In Situ Hybridization (Cells)
Normal interphase cells as indicated by two red/green fusion signals per nucleus.
This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq
Other Designations:
RET transforming sequence,cadherin family member 12,hydroxyaryl-protein kinase,oncogene RET,receptor tyrosine kinase,ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease)
Gene Pathway
Endocytosis
Pathways in cancer
Thyroid cancer
Related Disease
Adenocarcinoma
Adenocarcinoma, Follicular
Adenocarcinoma, Papillary
Adenoma
Adrenal Gland Neoplasms
Alzheimer Disease
Atherosclerosis
Atherosclerosis
Carcinoma
Carcinoma, Medullary
Carcinoma, Papillary
Carcinoma, Papillary, Follicular
Cleft Lip
Cleft Palate
Digestive System Abnormalities
Disease Progression
Down Syndrome
Endocrine Gland Neoplasms
Esophageal Neoplasms
... see more
Genetic Predisposition to Disease
Glomus Tumor
Hashimoto Disease
Head and Neck Neoplasms
Hippel-Lindau Disease
Hirschsprung Disease
Hydronephrosis
Hyperplasia
Hypertension
Intestinal Diseases
Kidney Diseases
Lung Neoplasms
Lymphatic Metastasis
Macular Degeneration
Multiple endocrine neoplasia
Multiple Endocrine Neoplasia Type 2a
Multiple Endocrine Neoplasia Type 2b
Necrosis
Neoplasm Metastasis
Neoplasm Recurrence, Local
Neoplasms
Neoplasms, Multiple Primary
Neoplasms, Second Primary
Neurofibromatosis
Nondisjunction, Genetic
Pancreatic cancer
Pancreatic Neoplasms
Paraganglioma
Parkinson Disease
Parkinson disease
Pheochromocytoma
Prostate cancer
Prostatic Neoplasms
Pulmonary Disease, Chronic Obstructive
Recurrence
Schizophrenia
Sleep Apnea, Obstructive
Sudden Infant Death
Syndrome
Taste
Thyroid Diseases
Thyroid Neoplasms
Tobacco Use Disorder
Urea Cycle Disorders, Inborn
Urinary Bladder Neoplasms
Urogenital Abnormalities
Vesico-Ureteral Reflux
Vitamin A Deficiency
von Hippel-Lindau Disease
Von Hippel-Lindau syndrome
Werner syndrome
... hidden
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