Bovinepancreaticdeoxyribonuclease(DNase)isanendonuclease,whichsplitsphosphodiesterlinkagespreferentiallyadjacenttoapyrimidinenucleotide,yieldingpolynucleotideswithafreehydroxylgroupatthe3'positionandaphosphategroupatthe5'position.TheoptimumpHis7.8.DNaseIisactivatedbybivalentmetalsandinhibitedbychelatingagentssuchasEDTAandsodiumdodecylsulfate.
ThisproductisRNasefree.
ProductSpecifications
DNaseI
DeoxyribonucleaseI
DNaseI,BovinePancreas,>1800KunitzU/mg,RNaseFree
UltraPureGrade
UnitDefinition:OneKunitzUnitwillproduceanA260of0.001perminuteatpH5.0at25°CusingDNA,typeIorIIIasthesubstrate.
Storage/Handling:Storedesiccatedat-20°C.
Allowproducttocometoroomtemperaturebeforeopening.
ForgreateststABIlity,itisimportantthatDNasetobedissolvedataconcentrationofatleast1mg/mlin50%Glycerolwith20mMTris-HCl,pH7.5,and1mMMgCl2.