The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys5.
Immunogen Range
Jan-50
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
Specificity
Histone H2B (Acetyl-Lys5) Antibody detects endogenous levels of total Histone H2B protein only when acetylated at Lys5.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
H2BFS
Database Links
Gene ID: 54145 Swiss-Prot #: P57053
Unigene #: Hs.473961
Target Modification
Acetyl
Modification Sites
H:K5 M:K5
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys5) Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Acetyl-Lys5) Antibody. The picture on the right is blocked with the synthesized peptide.