Histone H2B (Acetyl-Lys12) Antibody detects endogenous levels of total Histone H2B protein only when acetylated at Lys12.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
H2BFS
Database Links
Gene ID: 54145 Swiss-Prot #: P57053
Unigene #: Hs.473961
Target Modification
Acetyl
Modification Sites
H:K12 M:K12
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H2B (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.
Image 3
Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using Histone H2B (Acetyl-Lys12) Antibody. The lane on the right is blocked with the synthesized peptide.