The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Histone H4 (Acetyl-Lys16) Antibody
Synonyms
H4F2; HIST; HIST1H4A; Histone H4
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys16.
Immunogen Range
15-64
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
Specificity
Histone H4 (Acetyl-Lys16) Antibody detects endogenous levels of total Histone H4 protein only when acetylated at Lys16.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys16) Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H4 (Acetyl-Lys16) Antibody. The picture on the right is blocked with the synthesized peptide.