The SafeRed? Loading Dye is used for running DNA samples on agarose gel. The SafeRed? Loading Dye contains two regular dyes (Xylene Cyanol FF and Bromophenol Blue), and a safe, non-carcinogen red fluorescent dye. The Xylene Cyanol FF and Bromophenol Blue are used for monitoring the agarose gel while it is running, and the red fluorescent dye is to replace the toxic carcinogen ethidium bromide (EtBr). During the gel electrophoresis, the agarose gel will reveal a purple band and a blue band. The gel can be directly visualized under UV light without any extra staining steps.