| Description: | ExoFast? Exosome Isolation Reagent from plasma enables fast and efficient enrichment of intact exosomes from plasma samples. Isolation of exosomes can be a tedious, non-specific, and difficult process. ExoFast? Exosome Isolation Reagent from plasma enables simple and reliable concentration of intact exosomes from plasma samples with a protocol that is scalable depending on your sample size. By tying up water molecules, the reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. After a 10 minute treatment with Proteinase K, the reagent is added to the plasma sample, and the solution is incubated?at 2°C to 8°C for 30 min. The precipitated exosomes are recovered by standard centrifugation at 10,000 x g for 5 min at room temperature. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods. |
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