The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
K8 (Acetyl-Lys483) Antibody
Synonyms
KRT8; CYK8; Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
Immunogen
The antiserum was produced against synthesized Acetyl-peptide derived from human K8 around the Acetylation site of Lys483.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC 1:50~100 ELISA: 1:10000
Specificity
K8 (Acetyl-Lys483) Antibody detects endogenous levels of K8 protein only when acetylated at Lys483.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
KRT8
Database Links
Gene ID: 3856 Swiss-Prot #: P05787
OMIM #: 148060 Unigene #: Hs.533782/Hs.708445
Target Modification
Acetyl
Modification Sites
H:K483
Application Images
Image 1
Western blot analysis of lysate from A549 cells, using K8 (Acetyl-Lys483) Antibody.