The antiserum was produced against synthesized Acetyl-peptide derived from human NFκB-p65 around the Acetylation site of Lys218.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC 1:50~100 ELISA: 1:10000
Specificity
NFκB-p65 (Acetyl-Lys218) Antibody detects endogenous levels of NFκB-p65 protein only when acetylated at Lys218.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RELA
Database Links
Gene ID: 5970 Swiss-Prot #: Q04206
OMIM #: 164014 Unigene #: Hs.502875
Target Modification
Acetyl
Modification Sites
H:K218, M:K218, R:K218
Application Images
Image 1
Western blot analysis of lysate from Jurkat cells, using NFκB-p65 (Acetyl-Lys218) Antibody.