產(chǎn)品概述:產(chǎn)品參數(shù) 外觀:可溶于 DMF 或 DMSO 的橘色固體 Ex/Em (MeOH) = 493/516 nm CAS 號:70363-83-6 分子式:C27H40N4O6 分子量:516.63儲存條件 -20℃避光保存,有效期見外包裝。 產(chǎn)品介紹 DiBAC4(3) 是一種慢響應(yīng)膜電位探針,它進入到去極化的細胞,和細胞內(nèi)的蛋白或膜結(jié)合,發(fā)出很強的熒光。細胞去極化導(dǎo)致染料內(nèi)流,熒光增強;相反,細胞超極化則熒光減弱。注意事項 1. 熒光染料均存在淬滅問題,請盡量注意避光, 以減緩熒光淬滅。 2. 為了您的安全和健康,請穿實驗服并戴一次性手套操作。
說明書:UE-D4008 MSDS:MSDS D4008 DiBAC4(3)
使用本產(chǎn)品的文獻:參考文獻1.Assessment of E. coli and Salmonella viability and starvation by confocal laser microscopy and flow cytometry using rhodamine 123, DiBAC4(3), propidium iodide, and CTC.應(yīng)用方向:測定大腸桿菌和沙門氏菌饑餓時的膜去極化2.Usefulness and limitation of DiBAC4(3), a voltage-sensitive fluorescent dye, for the measurement of membrane potentials regulated by recombinant large conductance Ca2+-activated K+ channels in HEK293 cells.應(yīng)用方向:檢測10微米Evans藍(BK通道開放劑)引起的膜超極化3.The calcium response to the excitotoxin kainate is amplified by subseqUEnt reduction of extracellular sodium.應(yīng)用方向:研究小腦顆粒細胞膜電位的變化4.Cytochrome P-450 metabolites but not NO, PGI2, and H2O2 contribute to ACh-induced hyperpolarization of pressurized canine coronary microvessels.應(yīng)用方向:研究冠狀微血管超極化5.Effect of u.v. light irradiation, starvation and heat on Escherichia coli beta-D-galactosidase activity and other potential viability parameters.應(yīng)用方向:研究大腸桿菌細胞膜電位6.Bending the primary cilium opens Ca2+-sensitive intermediate-conductance K+ channels in MDCK cells.應(yīng)用方向:測定MDCK細胞膜電位差的變化