ResultsSandwich ELISASandwich ELISA performed with CD45 recombinant protein and CD45 monoclonal antibody.ImmunocytochemistryICC staining performed on WBC using CD45 monoclonal antibody, HRP conjugated anti-rabbit secondary antibody and DAB (brown color). Hematoxylin was used as a counterstain. PC3 as the negative control.
Recommend Usage:
Immunocytochemistry (1:400-1:800)LiquidCell™ SuperSlide™ (0.75 uL per slide)
Storage Buffer:
In 1x PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq
Other Designations:
CD45 antigen,T200 glycoprotein,T200 leukocyte common antigen,glycoprotein,leukocyte-common antigen,protein tyrosine phosphatase, receptor type, c polypeptide