Description:
??Q5?High-FidelityDNAPolymeraseisanultrahigh-fidelity,ThermostableDNApolymerasewith3′→5′exonucleaseactivity,fusedtoaprocessivity-enhancingSso7ddomaintosupportrobustDNAamplification.Withanerrorrate~280-foldlowerthanthatofTaqDNAPolymerase,Q5High-FidelityDNAPolymeraseisidealforcloningandcanbeusedforlongordifficultamplicons.TheQ5High-Fidelity2XMasterMixoffersrobust,high-fidelityperformanceinaconvenientmastermixformat.ThemixcontainsdNTPs,Mg++andaproprietarybroad-usebufferrequiringonlytheadditionofprimersandDNAtemplateforrobustamplification,regardlessofGCcontent.Whenusedattherecommended1Xfinalconcentration,theQ5High-FidelityMasterMixcontains2mMMgCl2.
Q5High-FidelityDNAPolymeraseisunliketypical,lowerfidelityPCRenzymes.Todeterminetheoptimalannealingtemperaturesforagivensetofprimers,useoftheNEBTmCalculatorishighlyrecommended.
Thiskitcontainsasufficientsupplyofmastermixandnuclease-freewatertoperform50polymerasechainreactions(NEB#E0555S)or200reactions(NEB#E0555L).Foraddedconvenience,gelloADIngdye,andaQuick-LoadDNALAdderarealsoincludedinthiskit.
??KitComponents
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
Q5®High-Fidelity2XMasterMix | -20 | 2X |
Nuclease-freeWater | -20 | |
Quick-Load®Purple2-LogDNALadder(0.1-10.0kb) | 4 | |
GelLoadingDye,Purple(6X) | 25 | 6X |
Notes:
ProductspecificationsforindividualcomponentsintheQ5High-FidelityPCRKitareavailableseparately.