Most eukaryotic mRNAs require a 7-methyl guanosine (m7G) cap structureat the 5´ end and a poly(A) tail at the 3´ end to be efficiently translated. TheHiScribe T7 ARCA mRNA Kit (with tailing) is designed for quick production ofARCA capped and poly(A) tailed mRNA in vitro. Capped mRNAs are synthesizedby co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA,NEB #S1411) using T7 RNA Polymerase. The transcription reaction can beset up easily by combining the ARCA/NTP mix, T7 RNA Polymerase Mix and asuitable DNA template. The kit also allows for partial incorporation of 5mCTP,Pseudo-UTP and other modified nucleotides into mRNA. After a brief DNase Itreatment to remove the template DNA, capped mRNA is poly(A) tailed withPoly(A) Polymerase. mRNAs synthesized with the kit can be used for celltransfection, microinjection, in vitro translation and RNA vaccines.ARCA is incorporated into mRNA exclusively in the correct orientation, generatingcapped mRNA that is more efficiently translated. Standard cap analogscan be incorporated in either direction resulting in only 50% of capped mRNAthat is functional in protein translation.
This product is related to the following categories:
RNA Synthesis Products
This product can be used in the following applications: