當(dāng)前位置 ： NEB >>> NEB/LunaScript? RT Master Mix Kit (Primer-free)/25 reactions/E3025S
NEB/LunaScript? RT Master Mix Kit (Primer-free)/25 reactions/E3025S
  • NEB/LunaScript? RT Master Mix Kit (Primer-free)/25 reactions/E3025S

NEB/LunaScript? RT Master Mix Kit (Primer-free)/25 reactions/E3025S

價(jià)格: 試用 市場(chǎng)價(jià): 0.00
貨號(hào): E3025S
品牌: NEB
規(guī)格
數(shù)量
庫(kù)存(0)
特別 提示
代購(gòu)產(chǎn)品：無(wú)質(zhì)量問(wèn)題不接受退換貨，下單前請(qǐng)仔細(xì)核對(duì)信息。
下單后請(qǐng)及時(shí)聯(lián)系客服核對(duì)商品價(jià)格，訂單生效后再付款。
資深產(chǎn)品顧問(wèn)
咨詢(xún)顧問(wèn)

全國(guó)免費(fèi)服務(wù)熱線(xiàn)

4000-520-616

  • 自營(yíng)商城 一站式服務(wù)
  • 廠家直采 剔除溢價(jià)
  • 品質(zhì)甄選 正品保證
  • 嚴(yán)控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說(shuō)明
  • 常見(jiàn)問(wèn)題
    • The LunaScript RT Master Mix Kit (Primer-free) includes an optimized 5X master mix containing all the necessary components for first strand cDNA synthesis, except primers. The mix features the thermostable Luna Reverse Transcriptase, which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation, along with dNTPs. In addition, the presence of a blue tracking dye provides a visual indicator for the RT step and downstream applications.The mix is compatible with random primers, oligo dT primers, and gene-specific primers, enabling maximum cDNA synthesis flexibility. The cDNA product generated by the LunaScript RT Master Mix (Primer-free) can be used in a variety of downstream applications. For real-time PCR quantitation, a mixture of random and dT primers is recommended to generate cDNA. The mix offers robust, linear and sensitive detection from 1 μg total RNA input down to low copies of RNA, similar to the LunaScript RT SuperMix Kit (NEB #E3010). To generate long or full-length cDNAs, oligo dT primers should be used. With a short incubation at 55°C for 10 minutes, the LunaScript RT Master Mix (Primer-free) can make cDNA products up to 9 kb. A No-RT control mix is provided for ease of setting up control reactions.
      Figure 1: Comparison of supermix, master mix and cDNA synthesis kit formats
      Figure 2:At just 13 minutes, LunaScript offers the shortest available first-strand cDNA synthesis protocol
      Comparison of recommended protocols for cDNA synthesis. Both LunaScript RT SuperMix Kit and LunaScript RT Master Mix Kit require the shortest reaction time and tolerate elevated temperatures, reducing complications from RNA secondary structure.
      Figure 3:LunaScript RT Master Mix (Primer-free) supports numerous applications
      By adding different primers including a Random Primer Mix, d(T))23VN oligos, or random hexamers, the LunaScript RT Master Mix can produce cDNA that is ideally suited for downstream applications such as RT-qPCR, RT-PCR, and RNA-seq studies.
      Figure 4:LunaScript RT Master Mix (Primer-free) offers robust 2-step RT-qPCR quantitation
      A. In the presence of Random Primer Mix (NEB #S1330), the LunaScript RT Master Mix (Primer-free) can deliver linear cDNA yields across RNA inputs from 1 µg to 100 pg with linearity similar to LunaScript (NEB #E3010) in a 2-step RT-qPCR workflow.
      B. For average transcripts, the use of d(T)23VN primers offers cDNA coverage similar to the Random Primer Mix. However, for long transcripts (e.g., SMG1), random primers often enable better coverage of the 5´ end of the target, as seen in the example in the lower righthand box where the RT-qPCR target was located at the 5´ end of the 16 kb SMG1 transcript; its detection by an anchored dT primer alone [d(T)23VN] gave delayed Cq values when compared to the performance using the Random Primer Mix (a mixture of random hexamers and anchored dT primer).
      Figure 5:Better performance of LunaScript RT Master Mix (Primer-free) in 2-step RT-qPCR quantitation
      A. Several first strand cDNA synthesis kits were used according to manufacturer’s recommendations to generate cDNA using the random primers provided with each product. Input included human total RNA from 1 µg – 100 pg. cDNA products were then evaluated by qPCR using eight targets varying in abundance, length and %GC. qPCR detection of the cDNA products was performed using the Luna Universal Probe qPCR Master Mix (NEB #M3004). Results were evaluated for efficiency and ΔCq, where ΔCq measures low input detection and lack of non-template control (NTC) amplification (ΔCq = average Cq of NTC - average Cq of lowest input). Green box indicates target performance criteria (Efficiency = 90–110%, ΔCq ≥ 3). More details on this visualization method can be found in the application note “Development of a high-throughput data analysis method for quantitative real-time PCR (qPCR).”
      B. Results from four targets above (two typical control genes and two others) were plotted to examine Cq as a function of input. LunaScript gave the earliest Cq values when compared to other commercial kits.
      Figure 6:LunaScript RT Master Mix (Primer-free) can generate a broad range of cDNA products for use in routine or high fidelity PCR
      LunaScript RT Master Mix (Primer-free) was used to synthesize cDNA from 1 µg of Jurkat total RNA in the presence of d(T))23VN primers under the standard reaction condition of 55°C for 10 min, 95°C for 1 min. Subsequent to cDNA synthesis, different PCR amplification reagents can be used for PCR detection. For routine application up to 5 kb, OneTaq 2X Master Mix (NEB #M0482) is recommended. For highest fidelity, Q5 Hot Start High-Fidelity 2X Master Mix (NEB #M0494) is recommended. For highest yield of long PCR amplification, LongAmp Taq 2X Master Mix (NEB #M0287) can be used (data not shown).
      This product is related to the following categories:
      Luna? qPCR & RT-qPCR Products,
      RT-PCR Products,
      Reverse Transcriptases & RT-PCR Products,
      cDNA Synthesis & Reverse Transcriptases Products
      This product can be used in the following applications:
      RT-PCR,
      cDNA Synthesis,
      Reverse Transcription (cDNA Synthesis),
      RT-PCR & cDNA Synthesis
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴(yán)謹(jǐn)態(tài)度，由螞蟻淘公司專(zhuān)業(yè)人員負(fù)責(zé)品控、采購(gòu)、物流、銷(xiāo)售、售后，保障正品優(yōu)質(zhì)。以“快速好省，為科研提供好產(chǎn)品、好價(jià)格”為理念，直接鏈接原廠家，從全國(guó)各地原制造商嚴(yán)格挑選188款科研精品，剔除品牌溢價(jià)，188生物新電商，把好的產(chǎn)品帶給科研！? 力求給你最優(yōu)質(zhì)的商品。
  • Q：生物188產(chǎn)品正品保障嗎？
    A：生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗(yàn)，在業(yè)界享有良好的口碑；自營(yíng)商城，直接從廠家采購(gòu)， 自己的團(tuán)隊(duì)負(fù)責(zé)國(guó)際物流和清關(guān)，中間沒(méi)有第三方，所有流程嚴(yán)格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進(jìn)行修改；客服電話(huà)：4000-520-616

    Q：可以開(kāi)發(fā)票嗎？
    A：本網(wǎng)站所售商品都是正規(guī)清關(guān)，均開(kāi)具16%正規(guī)發(fā)票，發(fā)票金額含配送費(fèi)金額，另有說(shuō)明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷(xiāo)售，付款后即可發(fā)貨,一般一周內(nèi)送達(dá)！

    Q：如何聯(lián)系商家？
    A：有任何問(wèn)題夠可以電話(huà)咨詢(xún)我們，全國(guó)24小時(shí)免費(fèi)服務(wù)熱線(xiàn)：4000-520-616 或聯(lián)系我們的在線(xiàn)客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯(cuò)了怎么辦？
    A：同個(gè)訂單購(gòu)買(mǎi)多個(gè)商品可能會(huì)分為一個(gè)以上包裹發(fā)出，可能不會(huì)同時(shí)送達(dá)，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線(xiàn)客服或者致電4000-520-616。

    Q：退換貨/維修需要多長(zhǎng)時(shí)間？
    A：一般情況下，退貨處理周期為客戶(hù)收到產(chǎn)品一個(gè)月內(nèi)（以快遞公司顯示簽收時(shí)間為準(zhǔn)），包裝規(guī)格、 數(shù)量、品種不符，外觀毀損、短缺或缺陷，請(qǐng)?jiān)谑盏截?4小時(shí)內(nèi)申請(qǐng)退換貨；特殊商品以合同條款為準(zhǔn)。

何為188

極簡(jiǎn)而嚴(yán)謹(jǐn)，我們僅銷(xiāo)售188款生物醫(yī)學(xué)科研用品，款款都是爆款；因?yàn)樯偎跃劢?，聚焦甄選每一款產(chǎn)品，聚焦服務(wù)每一位客戶(hù)!

關(guān)注我們 :

點(diǎn)擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號(hào)

獲取最新優(yōu)惠活動(dòng)通知