當前位置 ： NEB >>> NEB/Gibson Assembly? Cloning Kit/10 reactions/E5510S
NEB/Gibson Assembly? Cloning Kit/10 reactions/E5510S
  • NEB/Gibson Assembly? Cloning Kit/10 reactions/E5510S

NEB/Gibson Assembly? Cloning Kit/10 reactions/E5510S

價格: 試用 市場價: 0.00
貨號: E5510S
品牌: NEB
規(guī)格
數(shù)量
庫存(0)
特別 提示
代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯(lián)系客服核對商品價格，訂單生效后再付款。
資深產品顧問
咨詢顧問

全國免費服務熱線

4000-520-616

  • 自營商城 一站式服務
  • 廠家直采 剔除溢價
  • 品質甄選 正品保證
  • 嚴控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說明
  • 常見問題
    • Gibson Assembly was developed by Dr. Daniel Gibson and his colleagues atthe J. Craig Venter Institute and licensed to NEB by Synthetic Genomics, Inc. Itallows for successful assembly of multiple DNA fragments, regardless of fragmentlength or end compatibility. It has been rapidly adopted by the syntheticbiology community due to its ease-of-use, flexibility and suitability for large DNAconstructs.Gibson Assembly efficiently joins multiple overlapping DNA fragments in asingle-tube isothermal reaction (1,2). The Gibson Assembly Master Mix includesthree different enzymatic activities that perform in a single buffer:
      • The exonuclease creates single-stranded 3´ overhangs that facilitate theannealing of fragments that share complementarity at one end (overlap region).
      • The proprietary DNA polymerase fills in gaps within each annealed fragment.
      • The DNA ligase seals nicks in the assembled DNA.
      The end result is a double-stranded fully sealed DNA molecule that can serveas template for PCR, RCA or a variety of other molecular biology applications,including direct transformation. The method has been successfully used by Gibson’sgroup and others to assemble oligonucleotides, DNA with varied overlaps(15–80 bp) and fragments hundreds of kilobases long (1–2).To help select the best DNA assembly method for your needs, please use our Synthetic Biology/DNA Assembly Selection Chart.For help designing primers, please view our primer design video.

      Overview of the Gibson Assembly CloningMethod

      Overview of the Gibson Assembly Cloning Method

      Specification:

      10 μl of 2X Gibson Assembly Master Mix was incubated with 6 fragments(5 fragments of 400 bp and one of 2,780 bp, with 40 bp overlap, 0.05 pmoleach) in a final volume of 20 μl at 50°C for 60 minutes. NEB 5-alpha CompetentE. coli (NEB #C2987) were transformed with 2 μl of the master mix/fragmentmixture using the transformation protocol on page 12. Greater than 100white colonies were observed when 1/10 of the outgrowth was spread on anampicillin plate with IPTG/Xgal and incubated overnight.Overview of Gibson Assembly Cloning Kit Protocol:
      • Design primers to amplify fragments (and/or vector) with appropriateoverlaps
      • PCR amplify fragments using a high-fidelity DNA polymerase.
      • Prepare linearized vector by PCR amplification using a high-fidelity DNA polymerase or by restriction digestion.
      • Confirm and determine concentration of fragments and linearized vector using agarose gel electrophoresis, a NanoDrop™ instrument or other method.
      • Add fragments and linearized vector to Gibson Assembly Master Mixand incubate at 50°C for 15 minutes to 1 hour, depending on number offragments being assembled.
      • Transform into NEB 5-alpha Competent E. coli (provided) or use directly inother applications.
      This product is related to the following categories:
      DNA Assembly, Cloning and Mutagenesis Kits Products
      This product can be used in the following applications:
      Gibson Assembly®
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態(tài)度，由螞蟻淘公司專業(yè)人員負責品控、采購、物流、銷售、售后，保障正品優(yōu)質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優(yōu)質的商品。
  • Q：生物188產品正品保障嗎？
    A：生物188質量把控人員具有十年的從業(yè)經驗，在業(yè)界享有良好的口碑；自營商城，直接從廠家采購， 自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進行修改；客服電話：4000-520-616

    Q：可以開發(fā)票嗎？
    A：本網站所售商品都是正規(guī)清關，均開具16%正規(guī)發(fā)票，發(fā)票金額含配送費金額，另有說明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內送達！

    Q：如何聯(lián)系商家？
    A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯了怎么辦？
    A：同個訂單購買多個商品可能會分為一個以上包裹發(fā)出，可能不會同時送達，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

    Q：退換貨/維修需要多長時間？
    A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規(guī)格、 數(shù)量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

何為188

極簡而嚴謹，我們僅銷售188款生物醫(yī)學科研用品，款款都是爆款；因為少所以聚焦，聚焦甄選每一款產品，聚焦服務每一位客戶!

關注我們 :

點擊QQ聯(lián)系我們
生物188微信

關注188微信公眾號

獲取最新優(yōu)惠活動通知

  • 品質甄選，正品保證

  • 自營電商，廠家直采

  • 極簡主義，188精品