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NEB/NEBNext? Fast DNA Fragmentation & Library Prep Set for Ion Torrent?/E6285S/10 reactions
  • NEB/NEBNext? Fast DNA Fragmentation & Library Prep Set for Ion Torrent?/E6285S/10 reactions

NEB/NEBNext? Fast DNA Fragmentation & Library Prep Set for Ion Torrent?/E6285S/10 reactions

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    • Description:






      The NEBNext? Fast DNA Fragmentation & Library Prep Set for Ion Torrent? contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing on the Ion Torrent Sequencer (Life Technologies, Inc.). Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

      Functional Validation
      Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the Ion Torrent platform.
      DNA Library Preparation Workflow for Ion Torrent
      DNA Library Preparation Workflow for Ion Torrent


      Representative Results from Libraries Constructed Using NEBNext for Ion Torrent
      Representative Results from Libraries Constructed Using NEBNext for Ion Torrent

      0.5 μg of DNA from 3 different genomes with varying GC content were used to construct 200 bp and 400 bp libraries using the NEBNext Fast DNA Fragmentation and Library Prep Set for Ion Torrent, analyzed by the Agilent? Bioanalyzer?.

      Representative Ion Torrent Run Report
      Representative Ion Torrent Run Report
      A typical Ion Torrent run report for libraries made from E. coli (K12 MG1655 strain) genomic DNA using NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent. A. Alignment Summary. 72 Mb of data was generated, with an average genome coverage of 15.5 X, from approximately 0.5 Million 200 bp reads. B. Read length histogram from 200 bp run.

      Figure 1: Relative size distribution of Fragmented End Repaired DNA as?seen using the Bioanalyzer??2100 (Agilent Technologies, Inc.).
      Figure 1: Relative size distribution of Fragmented End Repaired DNA as seen using the Bioanalyzer? 2100 (Agilent Technologies, Inc.).
      1 μg of E. coli DNA was fragmented and end?repaired for 20 minutes at 25°C, followed by?10 minutes at 70°C.
      Figure 2: Final Library size distribution using AMPure XP Bead Size Selection.
      Figure 2: Final Library size distribution using AMPure XP Bead Size Selection.

      Figure 3: Final Library Size distribution using E-Gel Size Selection.
      Figure 3: Final Library Size distribution using E-Gel Size Selection.

      Lot Control

      The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

      Reagents Supplied

      The following reagents are supplied with this product:

      Store at (°C)Concentration
      NEBNext DNA Fragmentation Master Mix-20
      T4 DNA Ligase-20
      NEBNext DNA Library Primers for Ion Torrent?-201X
      Magnesium Chloride (MgCl2) (200 mM)-20
      NEBNext DNA Library Adaptors for Ion Torrent-20
      NEBNext DNA Fragmentation Reaction Buffer-2010X
      Bst 2.0 WarmStart? DNA Polymerase-20
      NEBNext Stop Buffer-2010X
      NEBNext Q5 Hot Start HiFi PCR Master Mix-202X
      T4 DNA Ligase Buffer for Ion Torrent-2010X
      TE Buffer-200.1X
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