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NEB/NEBNext? Ultra??DNA Library Prep Kit for Illumina?/E7370S/96 reactions
  • NEB/NEBNext? Ultra??DNA Library Prep Kit for Illumina?/E7370S/96 reactions

NEB/NEBNext? Ultra??DNA Library Prep Kit for Illumina?/E7370S/96 reactions

價(jià)格: ¥24540.00 市場(chǎng)價(jià): 40900.00

貨號(hào): E7370S-96reactions
品牌: NEB
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    • Description:







      ?

      TheNEBNext??Ultra?DNALibraryPrepKitforIlluminacontainsenzymesandbuffersthatareidealtoconvertasmallamountofDNAinputintoindexedlibrariesfornext-generationsequencingontheIlluminaplatform(Illumina,Inc).TheworkflowofNEBNextUltraDNALibraryPrepKitforIlluminaisveryuser-friendlyandfastwithminimalhands-ontime.Eachofthesecomponentsmustpassrigorousqualitycontrolstandardsandarelotcontrolled,bothindividuallyandasasetofreagents.

      UltraDNALibraryPreparationWorkflowforIlluminaUltra DNA Library Preparation Workflow for Illumina


      NEBNextUltra?DNAprovideshighlibraryyieldsevenwithlow?inputsanddifficultsamplesNEBNext Ultra? DNA provides high library yields even with low inputs and difficult samples

      Alllibrarieswerepreparedwith5ngofinputDNAandrunonanAgilentBioanalyzer?.
      A.E.coligenomicDNAlibraries.1:Ladder;2:LibrarypreparedusingNEBNextDNA?LibraryPrepMasterMixSet(E6040);3:LibrarypreparedusingNEBNextUltraDNA?LibraryPrepKit(E7370).With5nginputs,libraryyieldsaresignificantlyhigherusing?theNEBNextUltra?DNAKit.
      B.FFPEsample.HumanlungtumorgenomicDNAfroma66-yearoldmale,extractedfrom?a10-yearoldFFPEblock(Biochain,Inc.).1:LibrarypreparedusingNEBNextUltraDNA?LibraryPrepKit(E7370).2:InputDNA(highlydegradedFFPEgenomicDNA).
      Readdepthcorrelationshowsconsistenthighcoveragefor5ng-1μginputamounts
      Read depth correlation shows consistent high coverage for 5 ng - 1 μg input amounts
      LibrarieswerepreparedwithvariousamountsofhumanIMR90gDNA(5,50,and1000ng)andPCRamplified.APCR-freelibrarywasalsopreparedwith1000nghumanIMR90gDNA.Sequencecoverageof10kbwindowsofthehumangenomewasanalyzedonGalaxy,andPearsoncorrelationcoefficient(R2values)werecalculatedusingMicrosoft?Excel?.Alllibrariesarehighlycorrelatedwitheachother,independentofDNAinputaswellasPCRamplification.HighcoveragewasachievedforallDNAinputamounts.

      NEBNextUltraDNAKitsdisplaystrongperformancewithinputamountsaslowas5ng
      NEBNext Ultra DNA Kits display strong performance with input amounts as low as 5 ng

      InputDNA:IMR90genomicDNA(humanfibroblast).

      PFMismatchRate:Therateofbasesmismatchingthereferenceforallbasesalignedtothereferencesequence.

      PercentDuplication:Thepercentageofmappedsequencethatismarkedasduplicate.

      PercentChimeras:Thepercentageofreadsthatmapoutsideofamaximuminsertsizeorthathavethetwoendsmappingtodifferentchromosomes.

      ThisdataillustratesthestrongperformanceoftheNEBNextUltraDNALibraryPrepKitforIllumina,evenwith5ngofhumangenomicDNAinput.Mismatchrate,%duplicationand%chimerasarealllow,andsimilar,regardlessofinputamount.

      FunctionalValidation
      EachsetofreagentsisfunctionallyvalidatedtogetherthroughconstructionandsequencingofaDNAlibraryontheIlluminasequencingplatform.

      LotControl

      Thelotsprovidedaremanagedseparatelyandqualifiedbyadditionalfunctionalvalidation.Individualreagentsundergostandardenzymeactivityandqualitycontrolassays,andalsomeetstringentcriteriaintheadditionalqualitycontrolslistedoneachindividualcomponentpage

      ReagentsSupplied

      Thefollowingreagentsaresuppliedwiththisproduct:

      Storeat(°C)Concentration
      NEBNextEndPrepEnzymeMix-20
      NEBNextEndRepairReactionBuffer-2010X
      Blunt/TALigaseMasterMix-20
      NEBNext?LigationEnhancer-20
      NEBNext®Q5®HotStartHiFiPCRMasterMix-20
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