The NEBNext Ultra II FS DNA Module has been optimized to convert 100 pg - 0.5 μg of intact DNA to fragmented, end-repaired DNA having 5´ phosphorylated, 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595), and is part of the Ultra II FS DNA workflow.
For Illumina library construction, the NEBNext Ultra II FS DNA Moduleis designed for use with the following:
- NEBNext Ultra II Ligation Module (NEB #E7595)
- NEBNext Ultra II Q5® Master Mix (NEB #M0544)
- NEBNext Oligos for Illumina (NEB #E7335, #E7500, #E7710, #E7730, #E6609, #E7600 #E7535, #E7350)
For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance.
- This product is related to the following categories:
- DNA Fragmentation & RNA Fragmentation,
- Automation for NEBNext? NGS Library Prep,
- Next Generation Sequencing Library Preparation