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Millipore/ECM200 | QCM 3 μm Endothelial Cell Migration Assay Fibronectin, Colorimetric/ECM200/1 kitSufficient for 12 ass
  • Millipore/ECM200 | QCM 3 μm Endothelial Cell Migration Assay Fibronectin, Colorimetric/ECM200/1 kitSufficient for 12 ass

Millipore/ECM200 | QCM 3 μm Endothelial Cell Migration Assay Fibronectin, Colorimetric/ECM200/1 kitSufficient for 12 ass

價(jià)格: ￥5724.00 市場(chǎng)價(jià): 9540.00
貨號(hào): ECM200
品牌: Millipore
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    • Description
      CatalogueNumberECM200
      TradeName
      • QCM
      • Chemicon
      DescriptionQCM3μmEndothelialCellMigrationAssayFibronectin,Colorimetric
      OverviewAlsoavailable:CellComb?ScratchAssay!Getbiochemicaldatafromascratchassay!ClickHere

      Introduction
      Angiogenesisisafundamentalprocessinvolvingthegrowthofnewbloodvesselsfrompre-existingvessels.Itisimportantindevelopmentandwoundhealing,aswellaspathologicdiseasessuchasdiabeticretinopathyandcancer.Duringangiogenesis,endothelialcellsneedtomoveoutofexistingvessels,migrateintonewareas,proliferateandassembleintonewcapillaries.Themigrationofendothelialcellsisregulatedbymanyangiogenicfactorsandanti-angiogenicfactors.Itiscriticalforresearcherstounderstandthemechanismsofendothelialcellmigration.

      Millipore"s3μmQCM?EndothelialCellMigrationAssay–Fibronectin,ColorimetricprovidesaquickandefficientsystemtostudytheABIlityofcompoundstoinduceorinhibitendothelialcellmigration.Thisassayalsoallowsscreeningofpharmacologicalagents,evaluationofintegrinsorotheradhesionreceptorsresponsIBLeforendothelialcellmigration,analysisofgenefunctionintransfectedcells,anddeterminationofECMproteininvolvementincellmovement.

      Thisversatileassaypermitscountingofindividualmigratorycells,and,moreimportantly,allowsquantitativeanalysisbyopticaldensity(OD)usingastandardmicroplatereader.Thisconvenientassayallowslargescalescreeningandquantitativecomparisonofmultiplesamplesandincludesindividualmigrationcontrolsforeachsample.
      MaterialsRequiredbutNotDelivered1.PrecisionPipettes:sufficientforaliquotingcells.
      2.Harvestingbuffer:EDTAortrypsinbasedcelldetachmentbuffer,orothercelldetachmentformulationsasoptimizedbyindividualinvestigators.Millipore’sready-to-usenon-mammaliandetachmentsolution,Accutase?(Cat.No.SCR005)canalsobeused.
      3.Endothelialcells(forexample:HUVECcells).
      4.Endotheliumcellculturemediumappropriateforsubjectcells,suchasEGM-2(Endothelialcellgrowthmedia-2)
      5.QuenchingMedium:serum-freemedium,suchasDMEM,MEMetccontaining5%BSA.Mustcontaindivalentcations(Mg2+,Ca2+)sufficientforquenchingEDTAinharvestingbuffer.
      6.SterilePBSorHBSStowashcells.
      7.Distilledwater
      8.(Optional)Chemoattractantorpharmacologicalagentaddedtoculturemedium.
      9.Lowspeedcentrifugeandtubesforcellharvesting.
      10.CO2incubatorappropriateforsubjectcells.
      11.Hemocytometerorothermeansofcountingcells.
      12.Trypanblueorequivalentviabilitystain.
      13.Microplatereader(540-570nmdetection)orspectrophotometer.
      14.Sterilecellculturehood
      15.(Optional)Graduatedocular(calibrated),orautomatedmethodforcountingstainedcellsonamembrane.
      16.Shaker
      ProductInformation
      Components
      • 1.FibronectinTestPlate:One24-wellcultureplate,containing12humanFN-coatedBoydenchambers,sufficientfortheevaluationof12testsamples.
      • 2.BSAControlPlate:One24-wellcultureplate,containing12BSA-coatedBoydenchambers,sufficientfortheevaluationof12controls.
      • 3.CellStainSolution:Onevial-10mL
      • 4.ExtractionBuffer:Onevial-10mL
      • 5.24-WellStainExtractionPlate
      • 6.96-WellStainQuantitationPlate
      • 7.Swabs:50ea
      • 8.Forceps:1pair
      DetectionmethodFluorescentColorimetric
      StorageandShippingInformation
      StorageConditionsStorekitmaterialsat2°to8°Cforuptotheirexpirationdate.Donotfreeze.
      Applications
      ApplicationThisQCM3μmEndothelialCellMigrationAssay–Fibronectin,Colorimetricisidealforthestudyofendothelialcellmigrationinresponsetoanangiogenicstimulus.
      ApplicationNotesTheMilliporeQCM?3μmEndothelialCellMigrationAssay–Fibronectin,Colorimetricisidealforthestudyofendothelialcellmigrationinresponsetoanangiogenicstimulus.Thequantitativenatureofthisassayisespeciallyusefulforlargescalescreeningofpharmacologicagents.BSA-coatedcontrolchambersprovideanappropriatemigrationcontrol.The3μmporesizeinthisassayisoptimalforendothelialcellssuchasHUVEC,butnotsufficientforfibroblastmigration.TheMilliporeQCM?3μmEndothelialCellMigrationAssay–Fibronectin,Colorimetricassayisintendedforresearchuseonly;notfordiagnosticapplications.
      Eachkitprovidessufficientmaterialsfortheevaluationof12samples.
      BIOLOGicalInformation
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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