Overview | Millipore’sFlowCellect?DNADamage(HistoneH2A.X)andCellCycleAnalysisKitisdesignedtoidentifythelinkagebetweenDNAdamageinrelationtocellcyclecheckpointsinflowcytometryapplications.ThekitincludesadirectlyconjugatedAnti-phospho-HistoneH2A.X(Ser139)AlexaFluor?488antibodyplusaDNAdye(PropidiumIodide)whichallowstheresearchertoperformbivariateanalysistoidentifywhereDNAdamagetakesplacerelativetothepositioningwithinthecellcycle.Thekitincludesanoptimizedprotocolwithallofthenecessarycomponentstoprovidetheresearcheratrue“plugandplay”designfortheirresearchwhenstudyingDNAdamageandcellcyclecheckpoints.Thisimmunocytochemicalmethodisverysensitive,anditoffersanassaytoscreenanti-tumordrugsateveryphaseofthecellcycle.
Inall,acomprehensiveunderstandingofDNAdamageanditsassociationwithcellcyclebehaviorcanprovidetheresearcherwithusefulinformationwhichwillbeimportantininterpretingtheintrinsicnatureofcellproliferation,apoptosis,DNArepair,andassistinthedevelopmentofanti-neoplasticagents.Millipore’sFlowCellect?DNADamage(HistoneH2A.X)andCellCycleAnalysisKitisdesignedtoallowtheresearchertoeasilyidentify,discriminate,andquantitatecellsundergoingDNAdamagerelativetoitspositioningwithinthecellcycle.
AllFlowCellectkitsareoptimizedonguava?benchtopflowcytometers.FlowCellectkitscanbeusedonanyflowcytometerfollowingthesameprotocolprovidingresearchersareliableandfullyvalidatedsolutiontostudyDSBsandcellcycledistributionrightinthecomfortoftheirownlab.Thiskitcontainsoptimizedfixation,permeABIlization,wash,andflowbufferstoprovideresearcherswithacompletesolutionforbivariateanalysistoassessDNAdamageinrelationtopositioningwithinthecellcycle. |