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Millipore/FCCS025106 | FlowCellect? MAPK Activation Dual Detection Kit/FCCS025106/25 Tests / Kit
  • Millipore/FCCS025106 | FlowCellect? MAPK Activation Dual Detection Kit/FCCS025106/25 Tests / Kit

Millipore/FCCS025106 | FlowCellect? MAPK Activation Dual Detection Kit/FCCS025106/25 Tests / Kit

價(jià)格: ￥4164.00 市場價(jià): 6940.00
貨號: FCCS025106
品牌: Millipore
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    • Description
      CatalogueNumberFCCS025106
      TradeName
      • FlowCellect
      DescriptionFlowCellect?MAPKActivationDualDetectionKit
      OverviewMillipore’sFlowCellect?MAPKActivationDualDetectionkitincludestwodirectlyconjugatedantibodies,aphospho-specificAnti-phospho-ERK1/2(Thr202/Tyr204,Thr185/Tyr187)-PEandanAnti-ERK1/2-AlexaFluor?647conjugatedantibodytomeasuretotallevelsofERK.ThistwocolorflowcytometrykitisdesignedtomeasuretheextentofMAPKphosphorylationrelativetothetotalMAPKexpressioninanygivencellpopulation.Bydoingsuch,thelevelsofboththetotalandphosphorylatedproteincanbemeasuredsimultaneouslyinthesamecell,resultinginanormalizedandaccuratemeasurementofMAPKactivationafterstimulation.Moreover,simultaneousmeasurementofbothtotalandphospho-ERK1/2confirmstargetspecificityofthephosphorylationevent.Together,atotalandphosphoantibodyduoperformedinmultiplexprovidesanenhancedandmorereliabledetectionofthephospho:totalratiowithinamixedpopulation.
      AllFlowCellectkitsaresuppliedwithoptimizedfixation,permeABIlization,wash,andassaybufferstoprovideresearcherstheabilitytodetecttheactivationofERKinflowapplications.Sufficientreagentsareprovidedfor25two-colorsamples.Thekitincludesalloptimizedfluorescentlylabeledantibodiesandbuffersnecessaryforcellpreparationandanalysis.
      AlternateNames
      • FlowCellect
      BackgroundInformationErk(Extracellularsignal-RelatedKinase)isafamilyoftwo,highlyhomologousproteinsdenotedasErk1(p44,MAPK3)andErk2(p42,MAPK1)thatbothfunctioninthesamepathway.ThetwoproteinsareoftenreferredtocollectivelyasErk1/2orp44/p42MAPkinase.TheErkpathwayisconsideredtheclassical,canonicalMAPK(Mitogen-ActivatedProteinKinase)signalingpathway.Itisanevolutionarilyconservedpathwaythatcontrolsandisacriticalregulatorofthegrowthandsurvivalthroughthepromotionofcellproliferationandthepreventionofapoptosis.Erkisinvolvedinthecontrolofmanyfundamentalcellularprocessesincludingcellproliferation,survival,differentiation,apoptosis,motilityandmetabolism.Erkisactivatedbygrowthfactorstimulationofreceptortyrosinekinases(RTKs),GPCR,and/orintegrinstimulation.ThisactivatestheRas-Raf-MEK-Erkpathwaythatresultsinthephosphorylation/activationofErk1/2(p44/p42)ontheTxYmotif(Thr202/Tyr204andThr185/Tyr187forErk1&Erk2,respectively).

      Theregulationofalargenumberofcellularprocesses(suchascellproliferation/differentiation,cellsurvivalandapoptosis)isdependentontheactivationofMAPKsignalingpathway,whichcanbemeasuredbythephosphorylationofERK1/2.
      ProductInformation
      Components
      • 1.20XAnti-phospho-ERK1/2(Thr202/Tyr204,Thr185/Tyr187)PEConjugatedAntibody:(PartNo.CS203329)Onevialcontaining150μL.
      • 2.20XAnti-ERK1/2-AlexaFluor?647ConjugatedAntibody:(PartNo.CS205075)Onevialcontaining150μL.
      • 3.FixationBuffer:(PartNo.CS202122)Onebottlecontaining13mL.
      • 4.10XWashBuffer:(PartNo.CS202123)Onebottlecontaining13mL.
      • 5.5XAssayBuffer:(PartNo.CS202124)One55mLbottle
      • 6.1XPermeabilizationBuffer:(PartNo.CS203284)Onebottlecontaining13mL.
      DetectionmethodFluorescent
      StorageandShippingInformation
      StorageConditions4-8°Cforantibodiesandbuffers
      Applications
      ApplicationThisFlowCellectMAPKActivationDualDetectionKitforFlowCytometryincludes2conjugatedantibodies,phospho-specificAnti-phospho-ERK1/2(Thr202/Tyr204,Thr185/Tyr187)-PE&Anti-ERK1/2-AlexaFluor647tomeasuretotallevelsofERK.
      KeyApplications
      • FlowCytometry
      BIOLOGicalInformation
      HostMouse
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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