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Millipore/FCCS025153 | FlowCellect? DNA Damage Histone H2A.X Dual Detection Kit/FCCS025153/25 Tests / Kit
  • Millipore/FCCS025153 | FlowCellect? DNA Damage Histone H2A.X Dual Detection Kit/FCCS025153/25 Tests / Kit

Millipore/FCCS025153 | FlowCellect? DNA Damage Histone H2A.X Dual Detection Kit/FCCS025153/25 Tests / Kit

價格: ￥4164.00 市場價: 6940.00
貨號: FCCS025153
品牌: Millipore
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    • Description
      CatalogueNumberFCCS025153
      TradeName
      • FlowCellect
      DescriptionFlowCellect?DNADamageHistoneH2A.XDualDetectionKit
      OverviewMillipore’sFlowCellect?DualDetectionkitsareaseriesofflowcytometryproductswhichincludeapairofantibodiesthatbindtothesameprotein;onetodetecttotalproteinexpressionandanothertodetectthephosphorylatedformofthesametarget.Usingtwoparameteranalysis,wecanachievetargetspecificdetectionofphosphorylationand,bydoingso,eliminatefalsepositiveswhileenhancingthesignaltonoiseratio.

      FlowCellect?HistoneH2A.XDNADamageDualDetectionkitincludestwodirectlyconjugatedantibodies,aphospho-specificAnti-phospho-HistoneH2A.X(Ser139)-PerCPandanAnti-HistoneH2A.X-FITCconjugatedantibodytomeasuretotallevelsofHistoneH2A.X.ThistwocolorkitisdesignedtodetecttheextentofHistoneH2A.XpathwayactivationbymeasuringH2A.XphosphorylationrelativetothetotalH2A.Xexpressioninanygivencellpopulation.Bydoingsuch,thelevelsofboththetotalandphosphorylatedproteincanbemeasuredsimultaneouslyinthesamecell,resultinginanormalizedandaccuratemeasurementofH2A.Xactivationafterstimulation.Moreover,simultaneousmeasurementofbothtotalandphospho-HistoneH2A.Xconfirmstargetspecificityofthephosphorylationevent.Together,atotalandphosphoantibodyduoperformedinmultiplexprovidesanenhancedandmorereliabledetectionofthephospho:totalratiowithinamixedcellpopulation.UsingthisantibodypairprovidesasensitiveandvaluabletooltostudythefactorsthatinduceDNAdamageand/oraffectDNArepair,andallowonetoexplorethelinkagebetweenDNAdamage,cellcyclecheckpoints,andinitiationofapoptosis.
      AlternateNames
      • FlowCellect
      BackgroundInformationH2A.XisamemberofthehistoneH2Afamily.HistoneH2A.XresidesdownstreamoftheDNAdamagekinasesignalingcascade.PhosphorylationofHistoneH2A.Xatserine139isanimportantindicatorofDNAdamage.AsthelevelofDNAdamageincreases,thelevelofphosphoHistoneH2A.X(alsoknownasγH2AX)increases,accumulatingatthesitesofDNAdamage.ThisaccumulationofphosphoHistoneH2A.XisoftenusedtoindicatethelevelofDNAdamagepresentwithinthecell.H2AXisalsoresponsIBLeforrecruitingresponseproteinstothesiteofDNAdamageandmayplayaroleinDNArepair.

      InordertovalidatethatthelevelofH2A.Xactivationisaccurate,atotalH2A.XantibodyismultiplexedbyflowcytometrytoallowthetotalandphospholevelstobenormalizedsinceDNAcontentlevelsvarybasedonwhichstagecellsarewithinthecellcycle.HistonedoublesincontentduringthecellcycleatthesamerateDNAcontentdoubles.HavingatotalH2A.Xwillaccountforthisincrease,sothephosphorylatedlevelscanbeaccuratelydetermined.

      Inall,acomprehensiveunderstandingofHistoneH2A.XactivityandunderstandingtheconsequenceofitsactivationcanprovidetheresearcherwithusefulinformationwhichwillbeimportantinunderstandingtheintrinsicnatureoftheDNAdamageresponse.Millipore’sFlowCellect?HistoneH2A.XDNADamageDualDetectionKitisdesignedtoallowtheresearchertomonitorandaccuratelymeasurephospho-specificHistoneH2A.Xactivationinapopulationofcells.
      ProductInformation
      Components
      • 1.20XAnti-phospho-HistoneH2A.X(Ser139)-PerCP:(PartNo.CS206553)Onevialcontaining150μL.
      • 2.20XAnti-HistoneH2A.X-FITC:(PartNo.CS206429)Onevialcontaining150μL.
      • 3.FixationBuffer:(PartNo.CS202122)Onebottlecontaining13mL.
      • 4.10XWashBuffer:(PartNo.CS202123)Onebottlecontaining13mL.
      • 5.5XAssayBuffer:(PartNo.CS202124)One55mLbottle
      • 6.1XPermeABIlizationBuffer:(PartNo.CS203284)Onebottlecontaining14mL.
      DetectionmethodFluorescent
      StorageandShippingInformation
      StorageConditions4-8°Cforantibodiesandbuffers
      Applications
      ApplicationThisFlowCellectDualDetectionKitisdesignedtodetectDNAdamage.Thekitincludesapairofantibodiesthatbindtothesameprotein;onetodetecttotalH2A.Xexpression&anothertodetectthephosphorylatedformofH2A.X.
      KeyApplications
      • FlowCytometry
      BIOLOGicalInformation
      HostMouse
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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