mutaFISH™ CEP1wt DNA Probes is designed to identify human CEP1 amplification on dsDNA in cells using padlock probe and in situ rolling-circle amplification technology.
Supplied Product:
Content:1. mutaFISH™ CEP1wt DNA Probe2. Detection Probe-FITC
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ DNA Accessory Kit 2 for Cells (Catalog #: KA4928) which provides necessary reagents and enzymes for in situ restriction digestion, exonucleolysis, mutaFISH™ hybridization, ligation and amplification prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human PBMC cells. CEP1 was detected via green signal (FITC) and CEP17 was detected via orange signal (Cy3) .
Application Image
mutation specific, Fluorescence In Situ Hybridization (Cells)