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Abnova/mutaFISH™ BRAF V600E V600wt RNA Probes/1kit/FP0011
  • Abnova/mutaFISH™ BRAF V600E V600wt RNA Probes/1kit/FP0011

Abnova/mutaFISH™ BRAF V600E V600wt RNA Probes/1kit/FP0011

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貨號(hào): FP0011
品牌: Abnova
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  • 詳情
  • 使用說明
  • 常見問題
      • Specification
      • Gene Info
      • Pathway
      • Disease
      • Related Products
      • List All
      • Specification
      • Product Description:
      • mutaFISH™ BRAF V600E V600wt RNA Probes is designed to detect human BRAF V600E gene mutation on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
      • Supplied Product:
      • Content:1. RT BRAF V600 Primer2. mutaFISH™ BRAF V600E RNA Probe3. mutaFISH™ BRAF V600wt RNA Probe4. Detection Probe-Aqua 4315. Detection Probe-Texas Red X
      • Storage Instruction:
      • Store at -20°C.Aliquot to avoid repeated freezing and thawing.
      • Note:
      • We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
      • Protocol:
      • PDF DownloadProtocol Download
      • Technology:
      • mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
      • Comparison:
      • FISH Probes vs mutaFISH™ Probes
      • Fluorophore:
      • Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)Texas Red X (Excitation Peak (nm): 595; Emission Peak613)
      • Probe Position:
      • Regulatory Status:
      • For research use only (RUO)
      • Video:
      • Datasheet:
      • PDF DownloadDownload
      • Applications
      • mutation specific, Fluorescence In Situ Hybridization (Cells)
      • mutation specific, Fluorescence <em>In Situ</em> Hybridization (Cells)
      • mutaFISH™ staining was performed in situ in human Sk-HEP1 cells. BRAF V600E point mutation was detected via orange signal (Cy3), BRAF V600wt was detected via red signal (Cy5).
      • mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
      • mutation specific, Fluorescence <em>In Situ</em> Hybridization (FFPE Tissue)
      • mutaFISH™ staining was performed in situ in mouse FFPE BRAF 293T tissue. BRAF V600wt was detected via red signal (Texas Red X).
      • Application Image
      • mutation specific, Fluorescence In Situ Hybridization (Cells)
      • mutation specific, Fluorescence <em>In Situ</em> Hybridization (Cells)
      • enlarge
      • mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
      • mutation specific, Fluorescence <em>In Situ</em> Hybridization (FFPE Tissue)
      • enlarge
      • Gene Information
      • Entrez GeneID:
      • 673
      • Gene Name:
      • BRAF
      • Gene Alias:
      • B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1
      • Gene Description:
      • v-raf murine sarcoma viral oncogene homolog B1
      • Omim ID:
      • 115150, 164757, 211980
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
      • Other Designations:
      • 94 kDa B-raf protein,B-Raf proto-oncogene serine/threonine-protein kinase (p94),Murine sarcoma viral (v-raf) oncogene homolog B1
      • Gene Pathway
      • Acute myeloid leukemia
      • Bladder cancer
      • Chemokine signaling pathway
      • Chronic myeloid leukemia
      • Colorectal cancer
      • Endometrial cancer
      • ErbB signaling pathway
      • Focal adhesion
      • Glioma
      • Insulin signaling pathway
      • Long-term depression
      • Long-term potentiation
      • MAPK signaling pathway
      • Melanoma
      • mTOR signaling pathway
      • Natural killer cell mediated cytotoxicity
      • Neurotrophin signaling pathway
      • Non-small cell lung cancer
      • Pancreatic cancer
      • Pathways in cancer
      • Prostate cancer
      • Regulation of actin cytoskeleton
      • Renal cell carcinoma
      • Thyroid cancer
      • Vascular smooth muscle contraction
      • Related Disease
      • Abnormalities, Multiple
      • Adenocarcinoma
      • Adenocarcinoma, Follicular
      • Adenocarcinoma, Mucinous
      • Adenocarcinoma, Papillary
      • Adenoma
      • Adenoma, Oxyphilic
      • Adrenal Cortex Neoplasms
      • Alcoholism
      • Articulation Disorders
      • Astrocytoma
      • Brain Neoplasms
      • Breast cancer
      • Breast Neoplasms
      • Carcinoma
      • Carcinoma, Basal Cell
      • Carcinoma, Medullary
      • Carcinoma, Non-Small-Cell Lung
      • Carcinoma, Papillary
          • ... see more
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