mutaFISH™ ACTBwt RNA Probes is designed to detect human ACTB gene on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human SK-N-BE2 cells. ACTB wildtype was detected via green signal (FITC).
Application Image
mutation specific, Fluorescence In Situ Hybridization (Cells)
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Gene Information
Entrez GeneID:
60
Gene Name:
ACTB
Gene Alias:
PS1TP5BP1
Gene Description:
actin, beta
Omim ID:
102630
Gene Ontology:
Hyperlink
Gene Summary:
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations:
PS1TP5-binding protein 1,actin, cytoplasmic 1,beta actin,beta cytoskeletal actin
Gene Pathway
Adherens junction
Arrhythmogenic right ventricular cardiomyopathy (ARVC)