mutaFISH™ CXCL9wt RNA Probes is designed to detect human CXCL9 gene on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Supplied Product:
Content:1. RT CXCL9 Primer2. mutaFISH™ CXCL9wt RNA Probe3. Detection Probe-Texas Red X
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
Comparison:
FISH Probes vs mutaFISH™ Probes
Fluorophore:
Texas Red X (Excitation Peak (nm): 595; Emission Peak613)
Probe Position:
Regulatory Status:
For research use only (RUO)
Video:
Datasheet:
Download
Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in CXCL9 transfected 293 cells. CXCL9 was detected via red signal (Cy5).
mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
mutaFISH™ staining was performed in situ in mouse FFPE CXCL9 on 293T xenograft tissue. CXCL9 was detected via red signal (Texas red X).
Application Image
mutation specific, Fluorescence In Situ Hybridization (Cells)
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mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)