mutaFISH™ CXCL10wt RNA Probes is designed to detect human CXCL10 gene on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
Comparison:
FISH Probes vs mutaFISH™ Probes
Fluorophore:
Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)
Probe Position:
Regulatory Status:
For research use only (RUO)
Video:
Datasheet:
Download
Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in CXCL10 transfected 293 cells. CXCL10 was detected via orange signal (Cy3).
Application Image
mutation specific, Fluorescence In Situ Hybridization (Cells)