mutaFISH™ CK19wt RNA Probes is designed to detect human CK19 gene on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in MCF7 cells. CK19 gene was detected via green signal (FITC).
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mutation specific, Fluorescence In Situ Hybridization (Cells)
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Gene Information
Entrez GeneID:
3880
Gene Name:
KRT19
Gene Alias:
CK19,K19,K1CS,MGC15366
Gene Description:
keratin 19
Omim ID:
148020
Gene Ontology:
Hyperlink
Gene Summary:
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq
Other Designations:
40-kDa keratin intermediate filament,cytokeratin 19,keratin, type I cytoskeletal 19,keratin, type I, 40-kd