Description: | DNAzol Reagent is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and liquid samples of animal, plant, yeast, and bacterial origin. The DNAzol Reagent is a novel guanidine-detergent lysing solution which permits selective precipitation of DNA from a cell lysate. DNAzol Reagent provides a simple, reliable, and efficient DNA isolation method from a variety of samples. The DNAzol Reagent protocol is fast and permits isolation of genomic DNA from a large number of samples of small or large volumes. During the isolation, a biological sample is lysed (or homogenized) in DNAzol Reagent and the genomic DNA is precipitated from the lysate with ethanol. Following an ethanol wash, DNA is solubilized in water or 8 mM NaOH. The procedure can be completed in 10-30 min with DNA recovery of 70-100%. The isolated DNA can be used without additional purification for applications such as Southern analysis, dot blot hybridization, molecular cloning, and polymerase chain reaction (PCR). |