Labeled FISH probes for identification of gene translocation using Fluoresecent In Situ Hybridization Technique. (Technology)
Quality Control Testing:
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
Storage Instruction:
Store at 4°C in the dark.
Note:
Hybridization position of the probes on the chromosome.
Probe 1:Size:Fluorophore:Location:
BRAFApproximately 1050kbFITC7q34
Probe 2:Size:Fluorophore:Location:
LMO7Approximately 870kbTexas Red13q22.2
Origin:
Human
Source:
Genomic DNA
Notice:
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Regulatory Status:
For research use only (RUO)
Datasheet:
Download
Applications
Fluorescent In Situ Hybridization (Cell)
Protocol Download
Application Image
Fluorescent In Situ Hybridization (Cell)
BRAF
LMO7
Gene Information
Entrez GeneID:
673
Gene Name:
BRAF
Gene Alias:
B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1
Gene Description:
v-raf murine sarcoma viral oncogene homolog B1
Omim ID:
115150, 164757, 211980
Gene Ontology:
Hyperlink
Gene Summary:
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
This gene encodes a protein containing a calponin homology (CH) domain, a PDZ domain, and a LIM domain, and may be involved in protein-protein interactions. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene, however, the full-length nature of some variants is not known. [provided by RefSeq
Other Designations:
F-box only protein 20,F-box protein Fbx20,LIM domain only 7,OTTHUMP00000018512,OTTHUMP00000040819,zinc-finger domain-containing protein
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