Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.
Glyoxal beads allow a covalent binding of agarose to lysine amino groups (similar orientation to CNBr). Glyoxal agarose beads are ready to use and require no activation. The resin has a high amount of aldehydes groups (40-60 μmoles Glyoxal / ml gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand.
The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups and the direction/ orientation required for the binding to the support.
High & Very High Density Resin vs. Low & Very Low Density Resin? High/Very High binding capacity ? Good binding capacity? High immobilized enzyme stability? Immobilized enzyme stability? Possibility of multiple binding points? Minimum distortion of immobilized enzyme
Storage/Handling: Store at 4°C. Do not freeze.
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