This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity:
Human
Quality Control Testing:
Immunoprecipitation-Western Blot (IP-WB)Immunoprecipitation of ACO1 transfected lysate using mouse monoclonal anti-ACO1 and Protein A Magnetic Bead (U0007), and immunoblotted with rabbit polyclonal anti-ACO1.
Supplied Product:
Antibody pair set content:1. Antibody pair for IP: mouse monoclonal anti-ACO1 (300 ug)2. Antibody pair for WB: rabbit polyclonal anti-ACO1 (50 ul)
Storage Instruction:
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
MSDS:
Download
Datasheet:
Download
Applications
Immunoprecipitation-Western Blot
Protocol Download
Application Image
Immunoprecipitation-Western Blot
Gene Information
Entrez GeneID:
48
Gene Name:
ACO1
Gene Alias:
ACONS,IREB1,IREBP,IREBP1,IRP1
Gene Description:
aconitase 1, soluble
Omim ID:
100880
Gene Ontology:
Hyperlink
Gene Summary:
Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs).IREs are stem-loop structures found in the 5" UTR of ferritin mRNA, and in the 3" UTR of transferrin receptor mRNA.The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA.Thus, IREB1 plays a central role in cellular iron homeostasis.It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes. [provided by RefSeq
Other Designations:
OTTHUMP00000021176,OTTHUMP00000021177,OTTHUMP00000045233,aconitase 1,aconitate hydratase,citrate hydro-lyase,ferritin repressor protein,iron regulatory protein 1,iron-responsive element binding protein 1
Gene Pathway
Biosynthesis of alkaloids derived from histidine and purine
Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
Biosynthesis of alkaloids derived from shikimate pathway
Biosynthesis of alkaloids derived from terpenoid and polyketide