This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity:
Human
Quality Control Testing:
Immunoprecipitation-Western Blot (IP-WB)Immunoprecipitation of ACAD8 transfected lysate using rabbit polyclonal anti-ACAD8 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-ACAD8.
Supplied Product:
Antibody pair set content:1. Antibody pair for IP: rabbit polyclonal anti-ACAD8 (300 ul)2. Antibody pair for WB: mouse purified polyclonal anti-ACAD8 (50 ug)
Storage Instruction:
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Interspecies Antigen Sequence:
Mouse (89); Rat (73)
Datasheet:
Download
Applications
Immunoprecipitation-Western Blot
Protocol Download
Application Image
Immunoprecipitation-Western Blot
Gene Information
Entrez GeneID:
27034
Gene Name:
ACAD8
Gene Alias:
ACAD-8,FLJ22590
Gene Description:
acyl-Coenzyme A dehydrogenase family, member 8
Omim ID:
604773, 611283
Gene Ontology:
Hyperlink
Gene Summary:
Isobutyryl-CoA dehydrogenase (EC 1.3.99), encoded by the ACAD8 gene, catalyzes the third step of the degradation of the branched chain amino acid valine (Nguyen et al., 2002 [PubMed 12359132]).[supplied by OMIM