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Millipore/IHCR1005-6 | Anti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1/IHCR1005-6/6 mL
  • Millipore/IHCR1005-6 | Anti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1/IHCR1005-6/6 mL

Millipore/IHCR1005-6 | Anti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1/IHCR1005-6/6 mL

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貨號: IHCR1005-6
品牌: Millipore
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    • Description
      CatalogueNumberIHCR1005-6
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-TyrosineHydroxylaseAntibody,prediluted,cloneLNC1
      BackgroundInformationTyrosinehydroxylaseplaysanimportantroleinthephysiologyofadrenergicneurons.Itisthefirstandrate-limitingenzymeinvolvedinthebiosynthesisofthecatecholaminesDopamineandNorepinephrinefromtyrosine.THis,therefore,ausefulMarkerfordopaminergicandnoradrenergicneurons.TheenzymaticactivityofTHrequiresferrousionsascofactorsandisbelievedtoberegulatedbyphosphorylation.AtleastfourisoformsofhumanTHhavebeenidentifiedwhichresultfromalternativesplicing.
      ProductInformation
      FormatPurified
      PresentationLiquiddilutedinPBS,pH7.2withstABIlizers,0.2%Tween20,and0.1%ProClin300aspreservative.
      StorageandShippingInformation
      StorageConditionsMaintainat2-8°C.Refertovialforexpirationdating.
      Applications
      ApplicationAnti-TyrosineHydroxylaseAntibody,prediluted,cloneLNC1detectslevelofTyrosineHydroxylase&hasbeenpublished&validatedforuseinIH(P).
      KeyApplications
      • Immunohistochemistry(Paraffin)
      ApplicationNotesAntibodyispredilutedandreadytouseforImmunohistochemistryofformalin-fixed,paraffin-embeddedtissues.

      Pretreatment:HeatInducedEpitopeRetrieval(HIER).RecommendCitrateBuffer,pH6.0(Cat.No.21545).NosignalwasdetectedwithoutEpitoperetrieval.

      Incubation:30minuteswithIHCSelect?DetectionKits.

      TyrosineHydroxylasehasbeenpredilutedforuseastheprimaryantibodywithChemicon"sIHCSelect?DetectionKitsandProtocols(CatalogNos.DAB050,DET-HP1000,APR050,andDET-APR1000),butothersupplier"sIHCdetectionsystemsmaybeused.Foroptimizedprotocoldetails,visitwww.chemicon.comandselecttheprotocolslinkunderCat.No.IHCR1005-6.
      BIOLOGicalInformation
      ImmunogenTyrosineHydroxylasepurifiedfromPC12cells.
      EpitopeRecognizesanepitopeontheoutsideoftheregulatoryN-terminusoftyrosinehydroxylase.
      CloneLNC1
      HostMouse
      SpecificityTyrosineHydroxylase.Recognizesaproteinofapproximately59-63kDabyWesternblot.DoesnotreactwiththefollowingonWesternBlots:dopamine-beta-hydroxylase,phenylalaninehydroxylase,trytophanhydroxylase,dehydropteridinereductase,sepiapterinreductase,phenethanolamine-N-methyltransferase(PNMT).
      IsotypeIgG1κ
      SpeciesReactivity
      • Chicken
      • Human
      • Mouse
      • Monkey
      • Rat
      • Frog
      • Lizard
      • Vole
      • Zebrafish
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTyrosinehydroxylaseisinvolvedintheconversionoftyrosinetodopamine.Astherate-limitingenzymeinthesynthesisofcatecholamines,tyrosinehydroxylasehasakeyroleinthephysiologyofadrenergicneurons.
      GeneSymbol
      • TH
      • TYH
      • EC1.14.16.2
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P07101#Playsanimportantroleinthephysiologyofadrenergicneurons.
      COFACTOR:Fe(2+)ion.
      SIZE:528aminoacids;58524Da
      TISSUESPECIFICITY:Mainlyexpressedinthebrainandadrenalglands.
      DISEASE:SwissProt:P07101#DefectsinTHarethecauseofautosomalrecessiveSegawasyndrome[MIM:605407];alsoknownasDOPA-responsivedystonia.Typically,itbeginsinchildhoodoradolescencewithprogressivedifficultyinwalkingand,insomecases,spasticity.Somecasespresentwithparkinsoniansymptomsininfancyandarereferredtoasautosomalrecessiveinfantileparkinsonism.
      SIMILARITY:SwissProt:P07101##Belongstothebiopterin-dependentaromaticaminoacidhydroxylasefamily.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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