Ubiquitin Linkage Screening Kit is designed for two purposes: 1) to identify the ubiquitin lysine residue that is used to link the polyubiquitin chain conjugated on a protein substrate; 2) to determine whether a protein substrate is polyubiquitinated or conjugated with multiple monoubiquitin. A typical 20 μl reaction contains 100 nM UBE1, 2 μM E2 (not supplied), 50 μM Ub or Ub mutants, 4 mM ATP, 2 μM E3 (not supplied) and 2 μM substrate (no supplied) in the ubiquitination buffer.
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