| | CatalogNo.: K-1400
Description: This384well,fluorescencepolarization-based,competitiveassaymeasuresthephosphataseactivityofSHIP2whichhydrolyzesthe5-phosphatefromPtdInd(3,4,5)P3.Afterthephosphatasereactionsarecomplete,reactionproductsaremixedwithaPI(3,4)P2detectorproteinandthefluorescentPI(3,4)P2probe.Polarization(mP)valuesdecreaseasprobebindingtothePI(3,4)P2detectorisdisplacedbyPI(3,4)P2producedbyenzymaticactivityandtheamountofunboundfluorescentprobeinthemixtureincreases.
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