The Neutral Sphingomyelinase Activity Assay kit can detect sphingomyelinase at concentrations as small as 78 μU/mL. The kit provides all reagents to measure the Neutral Sphingomyelinase (nSMase) activity of 40 samples ran in duplicate. In this enzyme coupled assay, N-SMase catalyzes the hydrolysis of sphingomyelin into phosphorylcholine and ceramide. Alkaline phosphatase then catalyzes phosphorylcholine to choline. Choline is them oxidized by Choline oxidase to form hydrogen peroxide. Finally, DAOS plus 4-Aminoantipyrine (4 AAP) in the presence of hydrogen peroxide and peroxidase results in the oxidative coupling of DAOS and 4 AAP to form a blue chromogen that is detected by measuring the absorbance of light at 595 nm.
Order #:
K-1800-EC
Unit Size:
EA KIT
Supplier:
Echelon Biosciences
shipping:
See Manual
storage:
See Manual
restrictions:
Available in all European countries, except France & Italy.