The carboxyl terminus of Hsc70-interacting protein (CHIP) is an E3 ubiquitin ligase.CHIP contains two domains, one is its TPR domain on the amino terminus and the other is its U-box domain on the carboxyl terminus.The TPR domain recognizes the chaperones, Hsp70 and Hsp90.While the U-box domain carries out its ubiquitin ligase activity.CHIP often ubiquitinates misfolded proteins bound on the chaperones.