The antiserum was produced against synthesized peptide derived from mouse Caspase 9.
Immunogen Range
323-372
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
Caspase 9 (Cleaved-Asp353) Antibody detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp353.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
Casp9
Database Links
Gene ID: 58918 Swiss-Prot #: Q9R0S9/Q9JHK1
OMIM #: NA Unigene #: Mm.88829/Rn.32199
Target Modification
Cleaved
Modification Sites
353
Application Images
Image 1
Image 2
Western blot analysis of lysates from NIH/3T3 cells, treated with Etoposide 25uM 60', using Caspase 9 (Cleaved-Asp353) Antibody. The lane on the right is blocked with the synthesized peptide.